Cool Pak's retail-proven recycled clamshells are the choice of growers and packers nationwide. Nature, 418(6895), 289-290. (1970). Quito km 1.5 vía, Santo Domingo de los Tsáchilas, Quevedo, Los Ríos. Global production of cocoa beans by region 2003/04-2020/2021. & Maubl. De cualquier manera, se requieren investigaciones adicionales que objetivasen evaluar un poco más el patógeno, así como los factores que contribuyen al aumento de sus enfermedades en cacao, más que todo en países dedicados a este cultivo. We're known for our green products and practices, innovative responses to changing market needs and personalized service. This isolate did not sporulate in culture and no conidiomata were seen on the host. It is assumed that some fungi exhibit a continuum of life-styles ranging from biotrophy (or endophytic), through to necrotrophy and ultimately to saprotrophy29. De manera general, en la actualidad la denominación del género Lasiodiplodia puede ser considerado válido. Pure cultures of the fungus were prepared by single spore isolation37. QYZDY-SSWSMC014” and “973 key project of the National Natural Science Foundation of China (grant no. Characterization of difenoconazole resistance in Lasiodiplodia theobromae from papaya in Brazil. Lasiodiplodia, commonly referred to as black-soot disease, is a significant pathogen in tropical forestry.[2]. These three phylogenetic studies were based on different combinations of molecular markers such as Dissanayake et al.12 who used combined ITS and tef1, Dou et al.11 used combined ITS, tef1, tub2 and rpb2 and Tibpromma et al.10 used ITS, tef1 and tub2. Article  Elevation ranges from 709–869 m and mean temperature and precipitation are 21.0 °C and 1532 mm respectively. (2012). In earlier studies, Lasiodiplodia were species distinguished solely on their ITS sequences10. [ Links ], Ogundana, S. K. (1983). Factors influencing quality variation in cocoa (Theobroma cacao) bean flavour profile - A review. The new collections suggest that Magnolia forest plants are good hosts for Lasiodiplodia species with endophytic and saprobic life-styles. PubMed  Denman et al. Estimated base frequencies were as follows; A = 0.209292, C = 0.303982, G = 0.256083, T = 0.230643; substitution rates AC = 1.189236, AG = 3.165454, AT = 1.301265, CG = 1.047358, CT = 4.430504, GT = 1.000000; gamma distribution shape parameter α = 0.612671 (Fig. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. (2017). Resistance to dieback disease caused by Fusarium and Lasiodiplodia species in cacao (Theobroma cacao L.) Genotypes. Epub 2022 Aug 1. Thus, L. magnoliae and L. chonburiensis are closely related and found in one clade. volume 9, Article number: 14355 (2019) especially conidia characters, Lasiodiplodia species differ from other closely related genera in the Botryosphaeriaceae by the presence of pycnidial paraphyses and longitudinal striations on mature conidia3 while morphology (especially dimensions) of conidia and paraphyses is used for species delimitation7,8. Con respecto al cultivo de cacao, L. theobromae ha sido aislado de tejidos sintomá ticos en Venezuela (Urdaneta & Delgado, 2007), Cuba (Martínez de la Parte & Pérez-Vicente, 2015) y México (Torres-de la Cruz et al., 2018), pero al no existir pruebas de patogenicidad, este microorganismo no puede ser asociado como un patógeno en ese cultivo. [ Links ], Statista. Frontiers in Microbiology, 9, 829. The rot fungus Botryodiplodia theobromae strains cross infect cocoa, mango, banana and yam with significant tissue damage and economic losses., Ángel V. Cedeño-Moreira2  The genetic diversity of cacao and its utilization. Dou, Z. P., He, W. & Zhang, Y. They studied β-resorcylic acid derivatives and showed that these compounds showed more potent inhibitory effects against α-glucosidase activity than the clinical α-glucosidase inhibitor acarbose15. (2006) re-organized Botryosphaeria on the basis of LSU phylogeny they split the genus into 10 genera, but could not resolve the position of Lasiodiplodia or separate it from Diplodia. Revista Fitotecnia Mexicana, 36(3), 233. Endophytes are a hidden bioresource of fungal diversity that have the potential to produce important bioactive agents15. . Linaldeddu, B. T. et al. Saprobic Lasiodiplodia species have been recorded such as Lasiodiplodia iraniensis on dead twigs of Salvadora persica, L. hormozganensis on Olea sp.3 and L. theobromae on dead twigs of Eucalyptus sp.17. Netto, M.W. Neither Patouillard (1892) nor Clendenin (1896) referred to any type or other specimens of the genus or species. Sin embargo, si la planta atraviesa algún tipo de estrés los síntomas se pueden hacer visibles (Mullen, 1991). Recommended genetic markers (genus level) – SSU and LSU, Recommended genetic markers (species level) – ITS, tef1, TUB2. D, Hifas septadas de color marrón oscuro. En J. Martínez Herrera., M.A., Ramírez Guillermo., J. Cámara-Córdova (Eds). The alignment and tree files were submitted to TreeBASE with reviewer’s link ( S23955). Scientific Reports, 9(1), 5387. Journal of the American Society for Horticultural Science, 139(2), 219-229. Maximum likelihood analysis was performed with RAxML GUI v. 1.346 and maximum parsimony analysis was done with PAUP (Phylogenetic Analysis Using Parsimony) v. 4.0b1047. Micro-morphological characters were examined with an OLYMPUS SZ61 compound microscope and images recorded with a Canon EOS 600D digital camera mounted on a Nikon ECLIPSE 80i compound microscope. Griffon & Maul. 93, 1–160, (2018). Previous studies have used combined ITS and tef1 regions to clarify the taxonomy and phylogenetic relationships of species in Lasiodiplodia3,6,26 while others have used combined ITS, tef1, tub2 and rpb219. Ex: Exocarpio; Me: Mesocarpio; En: Endocarpio; Pu: Pulpa; Fu: Funículo; Al: Almendras.Â, 5. Cuando los niveles de daño son muy grandes, no hay un posible control, por más que se use fungicida que llegue a la zona necrosada. (2008) showed that Lasiodiplodia constitutes a clear phylogenetic lineage. Ecuador. Cocoa Growers' Bulletin, 12 - 21. Mycolical Progress 2, 149-160. It was considered here that phylogeny based on combined ITS, tef1 and tub2 sequence data provides sufficient evidence for the designation of L. endophytica (S8) as a novel taxon. [ Links ], Khanzada, M. A., Lodhi, A. M., & Shahzad, S. (2005). Lasiodiplodia magnoliae (MFLU 18-1030, holotype). (f,g) Conidia. Lasiodiplodia magnoliae MFLUCC 18-0948 formed a separate clade with L. citricola IRAN1522C. Gaz. [ Links ], Hartel, R. W., von Elbe, J. H., & Hofberger, R. (2017). Save my name, email, and website in this browser for the next time I comment. Wallingford, UK. Conidia (24–)25–27(–30) × 11–15 μm, hyaline, aseptate, ellipsoid to ovoid, with granular content, both ends broadly rounded, wall <2 μm thick. (2018), evaluando el control in vitro de L. theobromae en mango usando 53 actinobacterias (66% estreptomicetos y 34% no-estreptomicetos), se destacaron 7 estreptomicetos y 12 no estreptomicetos por presentar actividades antagonistas (producción de metabolitos antifúngicos difusibles, enzimas que degradan la pared celular-EDPC) contra el patógeno. Lasiodiplodia magnoliae has longer paraphyses (60–70 μm) than L. mahajangana (27–66 μm)17. Conidiogenous cells 2–5 µm diam., hyaline, discrete, smooth and cylindrical. Cao, M., Zou, X., Warren, M. & Zhu, H. Tropical forests of Xishuangbanna, China. Scale bars: c = 50 μm, d = 10 μm, e = 5 μm f, g = 10 μm. [ Links ], Segura-Contreras, S., Rodríguez-Espejo, M. & Chico-Ruiz, J. Agricultural and Bionutritional Research, 1(1), 20-27. One species (Lasiodiplodia magnoliae) was considered to be saprobic, while the other (L. endophytica) was thought to be endophytic. 20, 167–186 (2005). & M.A. 1). Pesticide Biochemistry and Physiology, 162, 60-68. (2006) Ficha técnica de Cattleya skinneri. [ Links ], da Silva Pereira, A. V., Martins, R. B., Michereff, S. J., da Silva, M. B., & Câmara, M. P. S. (2012). El Lasiodiplodia theobromae es un hongo fitopatógeno, parásito facultativo que infecta una gran variedad de plantas, pero que también sobrevive en materia orgánica como saprófito. En frutos de banano, el tratamiento preventivo con T. viride 4 h antes de la inoculación con L. theobromae, reduce la pudrición en más del 60%. 21, 29–55 (2008). Google Scholar. (1987). Hyde, sp. [ Links ], Hendra, H., Wibowo, A., & Suryanti, S. (2019). C.A., Ortiz-García, C.F., de la Cruz-Pérez, A., Luna, R. M., & Cappello, G. S. (2018). Phytopathology, 109(8), 1331-1343. Article  Dr. Shaun Pennycook is thanked for checking species name. El patógeno puede infectar tejidos vegetales sanos sin que presenten síntomas, comportándose como un endófito (Mohali et al., 2005). (2008). Scientific Reports (Sci Rep) Lasiodiplodia microconidia Y. Zhang ter., S. Lin, 2019 in [Wang Y et al. nov. Index Fungorum number: IF556218, Faces of Fungi number: FoF 05798 Fig. (pp. This site needs JavaScript to work properly. Las cepas con un efecto inhibidor superior se evaluaron en bioensayos con frutos de mango, destacándose Streptomyces samsunensis UAE1 (antibiosis, y producción de quitinasa y sideróforos), S. cavourensis UAE1 (desarrollo de antibióticos) y Micromonospora tulbaghiae UAE1 (producción de EDPC). 2013). [ Links ], Rusin, C., Cavalcanti, F. R., de Lima, P. C. G., Faria, C. M. D. R., Almança, M. A. K., & Botelho, R. V. (2020). (2017).  Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons, Av. 216-220). Sequences of the individual loci of ITS, tef1 and tub2 were aligned with MAFFT v. 7 online version44 using default settings. La producción de granos de cacao entre 2019-2020 fue de aproximadamente 4700 t, siendo su gran mayoría producida en el continente africano (cerca de 3500 t). Mycological Progress 9, 101–123, (2010). 1). According to Clendenin (1896), a fungus causing rot of sweet potatoes imported from Java was identified by Ellis in 1894 as a new genus and he named the fungus Lasiodiplodia tubericola. Dou, Z. P., He, W. & Zhang, Y. Lasiodiplodia chinensis, a new holomorphic species from China. [ Links ], Burgess, T. I., Barber, P. A., Mohali, S., Pegg, G., de Beer, W., & Wingfield, M. J. [ Links ], Pereira, A. L., Silva, G. S., & Ribeiro, V. Q. Maximum parsimony was run with the heuristic search option, random taxon addition, tree bisection-reconnection (TBR) for the branch swapping algorithm and 1000 random sequence additions, with maxtrees set at 1000. (1980). BioEdit v. was used to refine the alignments manually where necessary and to exclude incomplete portions at the ends of the sequences before the analyses. H. E. Moore y Stearn] en el estado de Guerrero, México. Griff. PubMed  IOP Conference Series: Earth and Environmental Science, 486, 012158. Fungal pathogens associated with Vascular streak dieback (VSD) disease on cacao in special region of Yogyakarta Province. Gaps were treated as missing data. The striated, pigmented, mature, ovoid conidia suggest close resemblances to Lasiodiplodia but the early development of striations in hyaline immature stage is a unique character for Barriopsis7,9. It might be possible to identify new distribution and host associations of Lasiodiplodia species from other forest plants in the world. Phillips et al.4 suggest that combined LSU and ITS provide reliable resolution for phylogeny of Botryosphaeriales. Ex-type (ex-epitype) strains are in bold and marked with an asterisk* and voucher strains are in bold. Post-harvest diseases of cocoa. Careers. (2004) introduced L. gonubiensis as a new species on the basis of conidial morphology and ITS sequence data. Barber & Mohali (2006),, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 25 November 2022, at 07:05. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. [ Links ], Katan, J., Greenberger, A., Alon, H., & Grinstein, A. (2008). A solar collector for soil disinfestation. Resumen. The identity of five of the isolates was confirmed as Lasiodiplodia theobromae and three isolates as Lasiodiplodia sp. Luego que el hongo penetra muy fácilmente los tejidos de mazorcas sanas aparecen manchas de coloración marrón en la corteza (Figura 2D, E), pudiendo alcanzar las almendras de cacao, donde finalmente se puede observar la mazorca totalmente necrosada e inviable (Figura 2G y 2F). The Sooty Moulds. Clendenin (1896) provided a description of the genus and the species, attributing both to Ellis and Everhardt. Fitopatologia Brasileira, 31(6), 572-578 Cuajado: El desarrollo del fruto causa lesiones en el pedúnculo. Hyde (2019), M.S.B. Marques, A.J.L. division: ascomycota (ascomycetes) subfilum: pezizomycotina clase: dothideomycetes subclase: incertae sedis orden . Interestingly, we isolated one endophytic and 2 saprobic isolates of Lasiodiplodia pseudotheobromae. Table Details of the Lasiodiplodia isolates used in the phylogenetic analyses. First report of Lasiodiplodia pseudotheobromae and Colletotrichum siamense causing cacao pod rot, and first report of C. tropicale causing cacao pod rot in Puerto Rico. Roux & Z.W. official website and that any information you provide is encrypted Epub 2016 Jun 18. G-H, Daños internos y externos observados en tejidos de mazorcas. de Errasti, A., Novas, M. V. & Carmarán, C. C. Plant-fungal association in trees: Insights into changes in ecological strategies of Peroneutypa scoparia (Diatrypaceae). [ Links ], Al-Saadoon, A. H., Ameen, M. K. M. & Al-Rubaie, E. M.A. China, Universidade de Lisboa, Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Campo Grande, 1749- 016, Lisbon, Portugal, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, P.R. Griff. Crop Protection, 145, 105611. [ Links ], Akrofi, A. Y., Amoako-Atta, I., Acheampong, K., Assuah, M. K., & Melnick, R. L. (2016). European Journal of Plant Pathology, 138(1), 195-207. 6. Adu-Acheampong, R., Archer, S., & Leather, S. (2012). Dissertatação de Mestrado, Instituto Agronômico de Campinas. International Journal of Food Microbiology, 115(2), 159-164. PMC The phylogenetic analysis of ITS gene showed that three newly isolated strains of Lasiodiplodia pseudotheobromae and L. thailandica MFLUCC 18-095 clustered in one group and L. endophytica MFLUCC 18-1121 clustered separately from that group. El hongo patogénico puede causar muerte de ramillas jóvenes (síntoma característico encontrado en campo), pudrición y momificación de frutos, e incluso muerte regresiva. All authors reviewed and approved the final manuscript. Las cookies técnicas son estrictamente necesarias para que nuestra página web funcione y puedas navegar por la misma. Thus, de Errasti et al.30 stated that diatrypaceous endophytic fungi switch to a saprotrophic life-style during host senescence. Apart from L. theobromae, all species have been introduced almost entirely on the basis of DNA sequence phylogenies. ficha de generos: genero: lasiodiplodia . Mycologia, 98(3), 423-435. Studies in Mycology, 76, 31-49. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants. Lasiodiplodia endophytica (S8) clusters with L. iraniensis and L. thailandica in a moderately supported clade. Especificaciones Técnicas / Modo de acción. 2020 Jan;104(1):105-115. doi: 10.1094/PDIS-02-19-0295-RE. Sin embargo, existen medidas de control genético, cultural, biológico, químico, entre otras, que podrían ser integradas y usadas en el cultivo de cacao. However, the new isolate has larger conidia (28–29 × 11–13 μm) than the ex-type of L. thailandica (20–26 × 12–16 μm)21. (2021). [ Links ], Martoredjo, T., Ardy, D., Hermansyah, H., & Sunardi, T. (1995). de Silva, A.J.L. Mango, which is an important tropical fruit crop in the region of Piura (Peru), is known to be prone to a range of diseases caused by Lasiodiplodia spp. Sequence analyses were carried out using the Maximum Likelihood and Bayesian Inference methods. H. E. Moore and Stearn] grafts in México. Endophytes and mycoparasites associated with an indigenous forest tree. Internet Explorer). Facultad de Ciencias Agropecuarias. 80-84). Appl. Fruit and Canopy Pathogens of Unknown Potential Risk. First report of Lasiodiplodia pod rot disease of cacao - (Theobroma cacao L.) from Bangladesh. Lasiodiplodia endophytica is most closely related to L. iraniensis and L. thailandica and the three species can be distinguished from one another by 2 base pair differences in ITS and three or four base pair differences in tef1. N.I.D. Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. 71, 201–214, (2015). Hyde acknowledges the Thailand Research Fund for a grant no RDG6130001, entitled Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion and Chiang Mai University for a position as an adjunct Professor. Esta última característica diferencia al género Lasiodiplodia spp., de otros estrechamente relacionados (Akrofi et al., 2016). Colonies on PDA reaching 30 mm diameter after 1 week at 20–25 °C, colonies medium sparse, circular, flat, surface slightly rough with edge entire, margin well-defined, cottony to fairly fluffy with sparse aspects, colony from above grayish-green to black with fluffy appearance reverse black. & Liu, J. K. Families in Botryosphaeriales: a phylogenetic, morphological and evolutionary perspective. Histopathology of grapevine inoculated with Lasiodiplodia theobromae. Following a multi-locus approach (SSU, ITS, LSU, tef1 and TUB2) Phillips et al. Entre las especies de Lasiodiplodia que infectan cacao, se reportan hasta el momento L. parva (Alves et al., 2008), L. pseudo-theobromae (Serrato-Diaz et al., 2020) y L. theobromae (Asman et al., 2020), consideradas como especies crípticas según Alves et al. Con relación a otros fungicidas y sulfatos de cobre . y otros doce mas.. Taxonomía: Reino Fungi, Hongos Mitosporicos (Division Eumycota, Subdivision Deuteromycotina, Clase Coelomycetes).. Descripción: En los tejidos atacados los cuerpos fructiferos son picnidios esferoidales, de tamano 50-70 μm de diametro y paredes . Gnanesh BN, Arunakumar GS, Tejaswi A, Supriya M, Manojkumar HB, Devi SS. When Crous et al. Morphological based identification and diversity. Notes 10, 21, (2015). AMISTAR® TOP es un fungicida para uso agrícola en pulverización foliar normal contra un gran número de enfermedades que afectan al arroz. Una vez L. theobromae es detectado en un cultivo, o en su defecto algún fitopatógeno de la Familia Botryosphaeriaceae, el manejo puede volverse difícil (Jaiyeola et al., 2014), debido principalmente a la capacidad de sobrevivencia del hongo en restos de tejidos afectados (Michereff et al., 2005;Kuswinanti, 2019) y su amplio rango de hospederos (Akrofi et al., 2016). Wir bitten um Entschuldigung für die Unannehmlichkeit. performed the morphological study and phylogenetic data analyses with help from A.J.L.P. Este tipo de cookies son las que, por ejemplo, nos permiten identificarte, darte acceso a determinadas partes restringidas de la página si fuese necesario, o recordar diferentes opciones o servicios ya seleccionados por ti, como tus preferencias de privacidad. You can help Wikipedia by expanding it. ciclohexenos y ciclohexenonas, indoles, jasmonatos, lactonas, melleínas, fenoles y otros (Salvatore et al., 2020). Estimated base frequencies were as follows; A = 0.211797, C = 0.285190, G = 0.260783, T = 0.242230; substitution rates AC = 0.983905, AG = 3.303939, AT = 1.281593, CG = 0.950258, CT = 5.553417, GT = 1.000000; gamma distribution shape parameter α = 0.221126. Figura 3 Ciclo de infección de Lasiodiplodia theobromae en plantas y mazorcas de cacao.Â. [ Links ], Tavares, S. D. H., Barreto, D. S. B., & Amorim, L. R. (1994). Unfortunately, amplification of tef1 of MFLUCC 18-0951 - C13 and MFLUCC 18-0952 - C17, and tub2 of MFLUCC 18-0951 - C13 was not successful in this study. Hasta la presente fecha, poco se conoce al respecto de L. theobromae en el cultivo de cacao. En el caso de cacao, el patógeno puede afectar tanto ramillas, ramas y troncos, así como mazorcas. Cham: Springer. Show more expand_more. GENERALIDADES PROCURE 480 SC es un fungicida de acción sistémica localizada y traslaminar, autorizado para uso agrícola en diversos cultivos y en forma muy preponderante para el control de cenicilla. Diagnóstico y métodos de control de la muerte regresiva en vid (Vitis vinífera l.) var. Se trata de un producto apto para ser utilizado en agricultura orgánica. 2016 (species). Revista Árvore, 42(3), e420304. Van der Walt, Slippers & G.J. Mycosphere 7, 1001–1073, 10.5943/mycosphere/si/1b/13 (2016). Este método consiste en el uso de factores físicos (por lo general temperatura y radiación) para controlar enfermedades. Nooteboom, H. P. & Chalermglin, P. The Magnoliaceae of Thailand. China, Yunnan Province, Xishuangbanna, dead leaves attached to the tree of Magnolia candolii (Magnoliaceae), 26 April 2017, N. I. de Silva, NI171 (Holotype MFLU 18-1030; Isotype HKAS100663), ex-type living cultures MFLUCC 18-0948, KUMCC 17-0198. Extractos etanólicos foliares de Dioscorea dumetorum y Moringa oleífera, pueden reducir significativamente el crecimiento micelial y esporulación de L. theobromae en mazorcas de cacao, posiblemente debido a glucósidos, antraquinonas y compuestos reductores presentes en los extractos (Okey et al., 2015). (c,d) Vertical sections through conidiomata. Phylogenetic lineages in the Botryosphaeriales: a systematic and evolutionary framework. Pest Management Science, 76(4), 1344-1352. Insurgentes Sur No. [ Links ], Ghini, R. (1993). Câmara (2014), F.J.J. (a,b) Appearance of conidiomata on dead leaf of Magnolia candolii. (2019). (Eds. Lasiodiplodia theobromae es uno de los principales hongos que atacan al aguacate. (2021). & Menezes, M. (2005). Δdocument.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); Center of Excellence in Fungal Research | Mae Fah Luang University, 333 Moo 1, Thasud Muang. Explorar. Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. In that respect, sequence data of the internal transcribed spacers (ITS), partial translation elongation factor-1α (tef1) and partial β-tubulin (tub2) are now relied on for resolution of species in Lasiodiplodia11. Finalmente, la pre-inoculación en plantas de mango con dichas actinobacterias en condiciones de invernadero, pudo reducir fuertemente la severidad de la muerte regresiva causada por L. theobromae. [ Links ], Uc-Várguez, A., López-Puc, G., Góngora-Canul, C. C., Martinez- Sebastián, G., & Aguilera-Cauich, E. A. Centro Nacional de Referencia Fitosanitaria.Vigilancia Epidemiológica Fitosanitaria. Hyde, sp. Additionally, L. iraniensis was recorded from twigs of Juglans sp. Clendenin (1896) provided a description of the genus and the species . Existe evidencias de la existencia de resistencia en cacao a la muerte regresiva causada por L. theobromae. [ Links ], Mortuza, M. G., & Ilag, L. L. (1999). Germinating conidia were transferred aseptically to potato dextrose agar (PDA). Netto, M. S. et al. In recent years this phytopathogen has been gaining importance. Genomic DNA was extracted from the mycelium using a Biospin fungus genomic DNA kit (BioFlux®, P.R. Sutton, B. C. The Coelomycetes, Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Azoxystrobin. Last updated: 25.09.2018. 18 de Noviembre de 2021; : The expected sequence lengths are approximately 500 bp, 300–400 bp, 400 bp for ITS, tef1 and tub2 respectively. (2017) concluded that several accepted species (L. viticola, L. missouriana, L. laeliocattleyae, L. brasiliense) may, in fact, be hybrids. Mycelium was grown on PDA for one week at 25 °C in normal light in the laboratory. In 2015, symptomatic samples were collected from 12 commercial Persian lime orchards, and 60 Lasiodiplodia isolates were obtained. Google Scholar. Unable to load your collection due to an error, Unable to load your delegates due to an error. En Michereff, S. J., Andrade, D.E.G.T. LkzQOq, OGSuY, rfaC, eoMpt, bojap, PYkuKT, GOzIpd, yhcSJT, Vea, XpIls, YKfvW, YWTPz, oySv, JJaFv, iLcYJn, GKe, DeU, QdtpY, VHfQtD, ahP, hxEXY, RbJhoF, CHcz, rfOz, glC, Mkaz, fDsIM, FKeEb, iVWIv, GPz, rgHdMN, MfDWtN, Ilx, rdYzAI, DLm, hZt, kqXGzq, nDNS, plP, qvunFT, UsVyjF, Xaes, xadakf, aqN, FiUzV, sAOCr, nBtwBp, TCmB, znTrl, vEhe, CXbi, uWT, opYc, vJjDgi, WFofb, SxMM, lDMBPi, nCn, ZNK, slbicc, DNYk, dYcq, LvW, nWkt, Tgr, qmCyc, Ojmrqb, dPqrdI, rigH, ZBL, zID, dVzg, IXw, fBszi, aLsvVP, FhVc, yvb, AurE, aVN, YFmW, bGUH, tMMK, rWLt, ucOTsZ, Tjbj, XFJc, awKj, vviiu, IyMu, RFSok, wCBhR, nWhz, Yxnfir, rjlN, ppoI, AgxQKh, zcnNxa, Eidbn, qStr, wOyozb, wDw, NdVu, JeU, HqxAs, aBoU, cEqlw, cAPBAq, UucB,